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Image Search Results
Journal: Cureus
Article Title: Investigation of the Use of Circulating Long Non-coding RNA HOXA Transcript at the Distal Tip (LncRNA HOTTIP) as a Biomarker in Breast Cancer
doi: 10.7759/cureus.50019
Figure Lengend Snippet: HOTTIP values (arbitrary units) In science and technology, an arbitrary unit or procedure defined unit is a relative unit of measurement that shows the ratio of amount of substance or other quantities to a predetermined reference measurement. HOTTIP: HOXA transcript at the distal tip, GAPDH: glyceraldehyde-3-phosphate dehydrogenase (used as an endogenous control for quantitative RT-PCR analysis because its expression is consistent at different time points and various experimental manipulations), CT: threshold cycle for each sample for each gene expression measured, ΔCT: CT HOTTIP - CT GAPDH, ΔΔCT: ΔCT sample - ΔCT pool, 2^(-ΔΔCT): standard strategy for qPCR data analysis based on the PCR efficiency
Article Snippet: The levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were estimated in the same samples using the respective predesigned primers - TaqMan probe set (
Techniques: Control, Quantitative RT-PCR, Expressing, Gene Expression
Journal: Frontiers in Physiology
Article Title: Tissue Nonspecific Alkaline Phosphatase (TNAP) Regulates Cranial Base Growth and Synchondrosis Maturation
doi: 10.3389/fphys.2017.00161
Figure Lengend Snippet: Alpl −/− primary chondrocytes exhibit abnormal gene expression during differentiation. (A–F) Primary rib chondrocytes isolated from Alpl +/+ and Alpl −/− mice were cultured to induce chondrocyte differentiation. Experiments were performed in triplicate. RNA was isolated at indicated time points. TNAP, sox9, aggrecan, Col2a1, Runx2, and Col10a1 mRNA levels were measured by real time PCR. Results are presented as normalized to β-Actin. Solid lines = Alpl +/+ cells, dashed lines = Alpl −/− cells. * p < 0.05 between genotypes.
Article Snippet: Real time PCR was performed utilizing the murine bActin primer/probe set 4352933E, the Sox9 primer/probe set Mm00448840_m1, the Col2a1 primer/probe set Mm01309565_m1, the Col10a1 primer/probe set Mm00487041_m1, the Runx2 primer/probe set
Techniques: Gene Expression, Isolation, Cell Culture, Real-time Polymerase Chain Reaction